Season 5

Shinpachi Shimura returns to the Yorozuya and discovers his friends drastically changed during the break. Feeling left behind, he returns home and discovers his sister, Tae Shimura, engaged to Isao Kondo. Tae and Kando enlists Shinpachi into the Shinsengumi and reveals that Tae is pregnant. Shinpachi runs to the ocean and laments the sudden change of those around him. There, he learns about Kyubei Yagyu's sex change to modify her life. They are interrupted by and Kotaro Katsura who also changed his genre due to his Kyubei's characters similarities.

As Katsura and Kyubei fight for the title of the best transgendered, Shinpachi leaves to find that the Shinsengumi members also suffered a sudden change in their selves; Sogo Okita is the leader from the group and orders him and Toshiro Hijikata, Sagaru Yamazaki to attack the Yorozuya. As the three leave, Hijikata knocks out Yamazaki, as he was faking his change. He and Shinpachi learn that an invasion of parasite aliens is responsible for change to their friends and proceed to destroy them. However, they find problems in the outcome, and both Hijikata and and Shinpachi are knocked out in the process, with Gintoki being actually only person without a parasite.

As Gintoki complains of writing News Years cards, Yorozuya learn of what his friends have done over the holidays. He also receives cards saying his friends have gotten married, while they have lied and just Photoshopped the photos. The episode ends with Kondo, Sachan and Hasegawa telling their story of how they wrote a News Years card. The second part starts with the same opening, but Gin complains of the lack of chocolate. Kagura has a chocolate for Gintoki and Shinpachi, but is too embarrassed to give to them. She enlists the help of Tsukuyo, and Sachan to help, but they are each too embarrassed to help her and make her give their chocolates to Gintoki. She indifferently give Gintoki and Shipachi the chocolate saying they were on the door step, as the happily run for the chocolate, they find all the chocolates were said to be from Kagura.

Yamazaki has a mental breakdown cause of Anpans and Kondo finally gets a date (Or does he!)

Catherine appears to have a boyfriend and has drastically improved her appearance. Her boyfriend is a regular at Snack Otose, an entrepreneur by the name of Suejiro. Catherine decides to leave the Kabuki district to start a new bar with him.

The Odd Jobs' TV breaks out with Sachan inside. Gintoki tells Sadaharu to throw her away with the TV, but Sachan's precious glasses are broken in the process. Gintoki reluctantly buys Sa-chan a new pair of glasses, but they are bottle bottom glasses that are clearly the wrong prescription. Sa-chan is overjoyed that Gintoki has given her a present, but the new glasses are responsible for a chain of failures on her assassination job. Her superiors now question her commitment.

Gintoki and crew continue to defend Sarutobi against the Lethal Punishers. But against such assassins as the Pillow Masa, T-shirt Tatsu, and the Chuubert Chu Brothers, will they win?

Due to a lack of budget, the Yorozuya try to use old footage to make a filler episodes. While Hasegawa first plays a theme song, the characters then watch a fake trailer a supposed second film based on the series. The episode ends with a chapter of "Teach us Ginpachi-sensei", a story in which Gintoki is the teacher from high school class composed of anime's characters.

Pirako Chin is looking for the strongest man in the Kabuki District. Now Gintoki has an underling who wants to turn Kabuki District into a garden of bright red flowers.

Pirako has double crossed Gin! Her real plan is to help Jirocho, her father, take over the town. Can Gin stop a war?

Pirako is pleased to hear that Otose, the reason for Jirocho's attachment to the Kabuki district, has been eliminated. With Otose gone, the balance of power among the Four Devas has crumbled and a war is in the works in the Kabuki district. Meanwhile, the Odd Jobs crew is watching over Otose when Saigo appears and informs them that the bar will be destroyed by the Four Devas. He urges them to leave this town, now that they've lost their home...Finally, the day comes for them to leave Snack Otose.

Tama, Tatsumi, Tetsuko, Kozen, Madao, Kyoshiro. Everybody is out in full force to fight for the Kabuki District. Male escorts vs. trannies, Cabaret girls vs. Yakuza, robots blowing things up. It’s an all-out war!